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Samples used as positive controls to validate the deep amplicon sequencing approach for the accurate detection of species composition.

Journal: Frontiers in Veterinary Science

Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

doi: 10.3389/fvets.2024.1491828

Figure Lengend Snippet: Samples used as positive controls to validate the deep amplicon sequencing approach for the accurate detection of species composition.

Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

Techniques: Amplification, Sequencing, Cell Culture

Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

Journal: Frontiers in Veterinary Science

Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

doi: 10.3389/fvets.2024.1491828

Figure Lengend Snippet: Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

Techniques: Sequencing

Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

Journal: Frontiers in Veterinary Science

Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

doi: 10.3389/fvets.2024.1491828

Figure Lengend Snippet: Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

Techniques: Sequencing